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Transmission electron micrograph of influenza A virus, late passage. Credit: CDC

甲型流感病毒的透射电子显微照片,晚期传播。

Lactic acid bacteria, commonly used as probiotics to improve digestive health, can offer protection against different subtypes of influenza A virus, resulting in reduced weight loss after virus infection and lower amounts of virus replication in the lungs, according to a study led by Georgia State University.

根据乔治亚州立大学(Georgia State University)领导的一项研究,通常用作改善消化系统健康的益生菌的乳酸菌可以针对不同亚型的甲型流感病毒提供保护,从而减少病毒感染后的体重减轻和肺部病毒复制的量。

Influenza virus can cause severe respiratory disease in humans. Although vaccines for seasonal influenza viruses are readily available, influenza virus infections cause three to five million life-threatening illnesses and 250,000 to 500,000 deaths worldwide during epidemics. Pandemic outbreaks and air transmission can rapidly cause severe disease and claim many more human lives worldwide. This occurs because current vaccines are effective only when vaccine strains and circulating influenza viruses are well matched.

流感病毒可引起人类严重的呼吸道疾病。虽然季节性流感病毒的疫苗很容易获得,但流感病毒感染在全球范围内造成了300万至500万的威胁生命的疾病和250,000至500,000的死亡。流行疫情和空气传播可能迅速导致严重疾病,并夺走世界上更多的人的生命。这是因为目前的疫苗只有在疫苗株和循环流感病毒很好地匹配时才有效。

Influenza A virus, which infects humans, birds and pigs, has many different subtypes based on hemagglutinin (HA) and neuraminidase (NA) proteins on the surface of the virus. There are 18 different HA and 11 different NA subtype molecules identified, which indicates numerous HA and NA influenza virus combinations. As a result, it's important to find ways to provide broad protection against influenza viruses, regardless of the virus strain.

甲型流感是一种感染人类、禽类和猪的病毒,它有许多不同的亚型,它是基于病毒表面的血凝素(HA)和神经氨酸酶(NA)蛋白。有18种不同的HA和11种不同的NA亚型分子,这表明有许多HA和NA流感病毒组合。因此,无论病毒株如何,都要找到方法来提供广泛的预防流感病毒的方法。

Fermented vegetables and dairy products contain a variety of lactic acid bacteria, which have a number of health benefits in addition to being used as probiotics. Studies have found some lactic acid bacteria strains provide partial protection against bacterial infectious diseases, such as Streptococcus pneumoniae, as well as cold and influenza viruses.

发酵的蔬菜和乳制品含有多种乳酸菌,除了被用作益生菌外,还对健康有益。研究发现,一些乳酸菌菌株可提供部分保护,预防细菌感染性疾病,如肺炎链球菌、感冒病毒和流感病毒。

This study investigated the antiviral protective effects of a heat-killed strain of lactic acid bacteria, Lactobacillus casei DK128 (DK128), a promising probiotic isolated from fermented vegetables, on influenza viruses.

本研究调查了一种热杀的乳酸菌的抗病毒保护作用,乳酸菌casei DK128(DK128),一种从发酵的蔬菜中分离出来的有前途的益生菌,用于流感病毒。

Mice pretreated with DK128 intranasally and infected with influenza A virus showed a variety of immune responses that are correlated with protection against influenza virus, including an increase in the alveolar macrophage cells in the lungs and airways, early induction of virus specific antibodies and reduced levels of pro-inflammatory cytokines and innate immune cells. The mice also developed immunity against secondary influenza virus infection by other virus subtypes. The findings are published in the journal Scientific Reports.

老鼠使用DK128鼻内感染甲型流感病毒显示,各种免疫反应与预防流感病毒,包括增加肺泡巨噬细胞细胞在肺部和呼吸道,早期诱导病毒特异性抗体水平,减少促炎细胞因子和先天免疫细胞。这些小鼠还对其他病毒亚型对继发性流感病毒的感染有免疫力。研究结果发表在《科学报告》杂志上。

"We found that pretreating the mice with heat-killed Lactobacillus casei DK128 bacteria made them resistant to lethal primary and secondary influenza A virus infection and protected them against weight loss and mortality," said Dr. Sang-Moo Kang, lead author of the study and professor in the Institute for Biomedical Sciences at Georgia State. "Our results are highly significant because mice pretreated with DK128 had 100 percent survival and prevention of weight loss. This strain of lactic acid bacteria also equipped mice with cross-protective immunity against secondary lethal infection with influenza virus. Protection against influenza virus infection was not specific to a particular strain of influenza.

“我们发现,预处理的老鼠heat-killed干酪乳杆菌DK128细菌使他们抵抗致命的主要和次要甲型流感病毒感染和保护他们对减肥和死亡率,”Sang-Moo Kang博士说,该研究的第一作者和生物医学科学研究所教授乔治亚州。“我们的结果是非常显著的,因为在DK128前治疗的小鼠能保持100%的存活率并防止体重下降。”这株乳酸菌也为小鼠提供了具有交叉免疫的抗流感病毒。预防流感病毒感染并非针对特定的流感株。

"Our study provides evidence that heat-killed lactic acid bacteria could potentially be administered via a nasal spray as a prophylactic drug against non-specific influenza virus infections."

“我们的研究提供了证据,证明热杀死的乳酸菌有可能通过鼻腔喷雾剂作为预防非特异性流感病毒感染的预防药物。”

The researchers pretreated mice intranasally with heat-killed DK128 and then infected them with a lethal dose of influenza A virus, subtype H3N2 or H1N1. Mice pretreated with a low dose of DK128 showed 10 to 12 percent weight loss, but survived the lethal infection of H3N2 or H1N1 virus. In contrast, mice pretreated with a higher dose of heat-killed DK128 did not show weight loss. Control mice, which were not pretreated with DK128, showed severe weight loss by days eight and nine of the infection and all of these mice died.

研究人员在鼻内用热杀死的DK128进行预处理,然后用致命剂量的甲型流感病毒、亚型H3N2或H1N1病毒感染它们。接受过低剂量DK128治疗的小鼠体重减轻10%至12%,但在H3N2或H1N1病毒的致命感染中存活下来。与此相反,用高剂量热杀DK128进行预处理的小鼠没有显示体重下降。对照组小鼠没有接受DK128的预处理,在第8天和第9天出现严重的体重下降,所有这些小鼠都死亡。

Mice that received heat-killed lactic acid bacteria (DK128) prior to infection had about 18 times less influenza virus in their lungs compared to control mice.

在感染前接受热杀乳酸菌(DK128)的小鼠,其肺部感染的流感病毒比对照小鼠低18倍。

Next, the researchers tested protection against secondary influenza virus infection by infecting pretreated mice with a different influenza A subtype from their primary virus infection. For the secondary virus infection, mice were exposed to H1N1 or rgH5N1.

接下来,研究人员通过将不同的甲型流感病毒(一种亚型)从原发性病毒感染中传染给经过预处理的小鼠,从而测试了对继发性流感病毒感染的保护作用。对于继发性病毒感染,小鼠暴露于H1N1或rgH5N1。

The study's results suggest that pretreatment with lactic acid bacteria, specifically DK128, equips mice with the capacity to have protective immunity against a broad range of primary and secondary influenza A virus infections.

该研究的结果表明,乳酸菌的预处理,特别是DK128,使小鼠具有对广泛的初级和继发性甲型流感病毒感染具有保护性免疫能力的能力。

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